ATF1 Antibody

• Catalog Number: orb1525572
• Category: Antibodies
• Description: ATF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 171 and 221
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ATF1
• UniProt ID: P18846
• NCBI: NP_005162.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Protein TREB36; TREB36; FUS/ATF-1; EWS-ATF1; cycli
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ATF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ATF1 antibody orb1525572 used for IP at 6 µg/mg lysate.

Detection of human ATF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ATF1 Cat. No. orb1525574 used at a dilution of 1: 200 (1µg/ml) (left) and Cat. No. orb1525572 used at a dilution of 1: 1, 000 (1µg/ml) (right). Detection: DAB. Counterstain: hematoxylin (blue).