ASPN antibody

• Catalog Number: orb18981
• Category: Antibodies
• Description: Goat polyclonal antibody to ASPN
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 43.3
• Target: Asporin / ASPN
• Entrez: 54829
• Protein Sequence: IHENKVKKIQKDT
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ASPN antibody, anti PLAP1 antibody, anti SLRR
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 40kDa band observed in Human Bone Marrow lysates (calculated MW of 43.4kDa according to NP_060150.3). Recommended concentration: 1-3 μg/ml.Experiment Notes: Immunofluoresence: in cell line ATDC5 shows signals in cytoplasm and on the cell surface. Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using ASPN antibody

Western blot analysis of human bone marrow lysate using ASPN antibody

Confocal immunofluoroscence analysis of ATDC5 cells using ASPN antibody

Western blot analysis of Human Tonsil(lane 1), Human Uterus(lane 2), Mouse Skeletal Muscle(lane 3), Rat Skeletal Muscle(lane 4) and Human Cerebellum(lane 5) lysate using ASPN antibody