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Catalog Number | orb2295777 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant ASNA1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 2H3 |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Rat |
Isotype | IgG1 Kappa |
Immunogen | ASNA1 (NP_004308, 239 a.a. ~ 348 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | PEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ |
NCBI | NP_004308 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ASNA1 monoclonal antibody (M03), clone 2H3 Western Blot analysis of ASNA1 expression in HeLa.
ASNA1 monoclonal antibody (M03), clone 2H3. Western Blot analysis of ASNA1 expression in PC-12.
Detection limit for recombinant GST tagged ASNA1 is approximately 0.03 ng/ml as a capture antibody.
Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10 ug/ml].
Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5 ug/ml].
Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody (M03), clone 2H3. Lane 1: ASNA1 transfected lysate(38.8 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody (M03), clone 2H3 (Cat # orb2295777). GAPDH (36.1 kDa) used as specificity and loading control.
Western Blot detection against Immunogen (37.84 KDa).