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Catalog Number | orb344610 |
---|---|
Category | Antibodies |
Description | ASK-1 (phospho-S83) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to an internal region near amino acids 75-100 of human ASK-1 protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:5,000 - 1:10,000, WB: 1:1,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product is an IgG fraction antibody purified from antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. Reactivity with the kinase from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q99683 |
NCBI | NP_005914.1 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-ASK1 pS83 Antibody, Apoptosis signal r Read more... |
Note | For research use only |
Application notes | This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimal reactivity by western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended. A 155 kDa band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 can be used as a positive control. For ELISA a 1:5,000 to 1:10,000 dilution is recommended. Researchers should determine optimal titers for other applications. |
Expiration Date | 12 months from date of receipt. |
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using ASK-1 (phospho-S83) antibody
Western blot analysis of Cos-7 cell lysates using ASK-1 (phospho-S83) antibody
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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