ASC/TMS1/Pycard Antibody

• Catalog Number: orb1728210
• Category: Antibodies
• Description: ASC/TMS1/Pycard Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Mouse
• Immunogen: E.coli-derived mouse ASC/TMS1/Pycard recombinant protein (Position: K24-S193).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5 μg/ml, Mouse Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse Flow Cytometry, 1-3 μg/1x1x106 cells, Mouse Direct ELISA, 0.1-0.5 μg/ml, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22 kDa
• UniProt ID: Q9EPB4
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of RAW264.7 cells using anti-ASC/TMS1/Pycard antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of ASC/TMS1/Pycard using anti-ASC/TMS1/Pycard antibody.Lane 1:mouse lung tissue;2:mouse spleen tissue;3:mouse thymus tissue.

IF analysis of ASC/TMS1/Pycard using anti-ASC/TMS1/Pycard antibody and anti-Tubulin Alpha antibody.ASC/TMS1/Pycard was detected in immunocytochemical section of RM1 cell.