ASAH1 monoclonal antibody (M02), clone 1A7

• Catalog Number: orb2295795
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant ASAH1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1A7
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: IgG3 Kappa
• Immunogen: ASAH1 (NP_808592, 25 a.a. ~ 124 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: PPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPMLKVIVNSLKNMINTFVPSGKVMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTD
• NCBI: NP_808592
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged ASAH1 is approximately 1 ng/ml as a capture antibody.

Immunoperoxidase of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml].

Western Blot analysis of ASAH1 expression in transfected 293T cell line by ASAH1 monoclonal antibody (M02), clone 1A7. Lane 1: ASAH1 transfected lysate(45 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (36.74 KDa).