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Catalog Number | orb18679 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ARPC2 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:32000, WB: 0.1-0.5 μg/ml, IHC-P: 10 μg/ml |
Conjugation | Unconjugated |
MW | 34.3 |
Target | ARPC2 |
Entrez | 10109 |
Protein Sequence | EMKTITGKTFSSR |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ARPC2 antibody, anti actin related protein 2/ Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5ug/ml.WB: Approx 33-34kDa band observed in Hela and Human Skeletal Muscle lysates (predicted MW of 34kDa according to NP_005722). Recommended for use at 0.1-0.5 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb18679 staining (0.2ug/ml) of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
orb18679 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis of CACO-2 (A), (0.1ug/ml) HeLa (B) and Jurkat (C) lysate using ARPC2 antibody
Immunofluorescence analysis of A431 cells using ARPC2 antibody
Immunofluorescence analysis of HeLa cells using ARPC2 antibody
ICC, IHC-P, WB | |
Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA | |
Human | |
Rabbit | |
Polyclonal | |
Biotin |
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