ARP2/3 subunit 1B/ARPC1B Antibody

• Catalog Number: orb546372
• Category: Antibodies
• Description: ARP2/3 subunit 1B/ARPC1B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ARP2/3 subunit 1B/ARPC1B recombinant protein (Position: Q293-K372).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 41 kDa
• UniProt ID: O15143
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Actin-related protein 2/3 complex subunit 1B; Arp2
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of CACO-2 cells using anti-ARPC1B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of PC-3 cells using anti-ARPC1B antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ARPC1B using anti-ARPC1B antibody.Lane 1:human placenta tissue;2:human PC-3 cell;3:human U-937 cell;4:rat spleen tissue;5:mouse spleen tissue.

IHC analysis of ARPC1B using anti-ARPC1B antibody.ARPC1B was detected in paraffin-embedded section of human tonsil tissues.

IHC analysis of ARPC1B using anti-ARPC1B antibody.ARPC1B was detected in paraffin-embedded section of mouse spleen tissues.

IHC analysis of ARPC1B using anti-ARPC1B antibody.ARPC1B was detected in paraffin-embedded section of rat spleen tissues.