Armenian Hamster IgG isotype Control Antibody

SKU: orb346446

Description

Armenian Hamster IgG isotype Control Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: FC, SDS-PAGE
• Dilution Range: FC: 1:1000-1:5000
• Application Notes:
  ARMENIAN HAMSTER IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by user.

Key Properties

• Purity: ARMENIAN HAMSTER IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Armenian Hamster IgG. Greatly diminished reactivity will occur against anti-Golden Syrian Hamster IgG.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Armenian Hamster IgG isotype Control, A. Hamster IgG control, Control Protein, Isotype Protein

Anti-CD154–facilitated alloengraftment is multilineage. Twenty mice from 2 representative experiments were phenotyped at 120 days after BMT for donor-host origin of CD4+ and CD8 + T cells, CD19 + B cells, and MAC-1 + myeloid cells. On the x-axis are shown the host and donor proportions of each of the lineages. ▪ indicates the proportion of each lineage of host origin; ▥, the proportion of each lineage that is of donor origin. On the y-axis is shown the percentage of PBLs of each lineage. Irrelevant hIgG–treated mice had no detectable donor chimerism and thus are composed entirely of host-type cells. Note that most CD4+ T cells, CD19 + B cells, and MAC-1 + myeloid cells in anti-CD154–treated mice are of donor origin. In contrast, most of the CD8 + T cells are of host origin. irrelevant hamster IgG (hIgG) (p/n orb346446).

SDS PAGE Results of Armenian Hamster IgG Whole Molecule Isotype Control. Lane 1: Armenian Hamster IgG Reduced [5.0 µg]. Lane 2: Armenian Hamster IgG Reduced [1.0 µg]. Lane 3: Lane 4: Armenian Hamster IgG Non-Reduced [1.0 µg]. Lane 5: Armenian Hamster IgG Non-Reduced [5.0 µg]. 4-20% Gel, Coomassie Stained.