ARK1C1 Antibody / Aldo-keto Reductase Family 1 Member C1

• Catalog Number: orb2635898
• Category: Antibodies
• Description: DDH / AKR1C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AKR1C1/9317
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant human full-length AKR1C1 protein was used as the immunogen for the ARK1C1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ARK1C1 antibody is available for research use only.
• UniProt ID: Q04828
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the ARK1C1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human A549 cell lysate using AKR1C1 antibody (clone AKR1C1/9317). Predicted molecular weight ~37 kDa.

Western blot testing of human HepG2 cell lysate using AKR1C1 antibody (clone AKR1C1/9317). Predicted molecular weight ~37 kDa.

IHC staining of FFPE human hepatocellular carcinoma tissue with AKR1C1 antibody (clone AKR1C1/9317). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free AKR1C1 antibody (clone AKR1C1/9317) as confirmation of integrity and purity.