ARID1B Antibody

• Catalog Number: orb1806439
• Category: Antibodies
• Description: ARID1B Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1400 and 1450
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000 (3-8% Tris-acetate gel recommended), IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ARID1B
• UniProt ID: Q8NFD5
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: CSS1; MRD12; Osa homolog 2; P250R; hOsa2; ELD/OSA1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ARID1B by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, U2OS, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of mouse ARID1B by western blot. Samples: Whole cell lysate (25 µg) from RenCa, EL4, A20, J774A1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse ARID1B/BAF250 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse ARID1B (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1B (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439) used for IP at 6 µl/mg lysate.

Detection of mouse ARID1B by immunocytochemistry. Sample: FFPE section of ND7/23 cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human ARID1B by immunocytochemistry. Sample: FFPE section of RKO cells. Antibody: Rabbit anti-ARID1B recombinant monoclonal antibody (orb1806439). Secondary: HRP-conjugated goat anti-rabbit IgG.