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Catalog Number | orb412189 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to ARHGAP5 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant full length protein of human RhoGAP5 |
Dilution range | WB: 1:500-2000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | ARHGAP5 |
Entrez | 394 |
UniProt ID | P97393, Q13017 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti-RHOGAP5 antibody, anti-Rho GTPase-activating Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of RhoGAP5 expression in Jurkat (A), HepG2 (B), PC12 (C), mouse brain (D), rat brain (E) whole cell lysates. (Predicted band size: 26; 27; 172 kD; Observed band size: 185 kD)
Immunofluorescent analysis of RhoGAP5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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