ARHGAP22 (phospho-S397) antibody

• Catalog Number: orb345738
• Category: Antibodies
• Description: ARHGAP22 (phospho-S397) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS397 region of ARHGAP22.
• Concentration: 1.07 mg/ml
• Dilution range: ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 1 µg/ml
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-ARHGAP22 pS397 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for phosphorylated ARHGAP22 at Serine 397. It also recognizes the S22->A mutation but not the S397->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS397 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q8BL80
• NCBI: NP_722495
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: rabbit anti-ARHGAP pS397 antibody, ARHGAP 22, ARHG
• Note: For research use only
• Application notes: Anti-ARHGAP22 pS397 antibody is tested for Western Blot and Immunostaining and useful for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NIH3T3 cells transfected with a null vector (Lane1), NIH3T3 cells transfected (Lane2), NIH3T3 cells transfected (Lane3), NIH3T3 cells transfected (Lane4) using ARHGAP22 (phospho-S397) antibody