Argonaute 2 Rabbit mAb [KD Validated]

• Catalog Number: orb1951233
• Category: Antibodies
• Description: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human Argonaute 2
• UniProt ID: Q9UKV8
• Tested applications: FC, ICC, IHC, IP, WB
• Dilution range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
• Antibody Type: Recombinant Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Ago 2; Argonaute 2; dAgo2; eIF2C 2; hAgo2; PPD;
• Note: For research use only

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of Argonaute 2 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.