ARF6 Antibody

• Catalog Number: orb371721
• Category: Antibodies
• Description: ARF6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Bovine, Gallus, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ARF6 recombinant protein (Position: G2-S175). Human ARF6 shares 100% amino acid (aa) sequence identity with both mouse and rat ARF6.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 20082 MW
• UniProt ID: P62330
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: ADP-ribosylation factor 6;ARF6;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-ARF6 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ARF6 using anti-ARF6 antibody.Lane 1:rat kidney tissue;2:mouse kidney tissue;3:MCF-7 cell.

IF analysis of ARF6 using anti-ARF6 antibody. ARF6 was detected in immunocytochemical section of A431 cells.

IHC analysis of ARF6 using anti-ARF6 antibody. ARF6 was detected in paraffin-embedded section of human melanoma tissue.

IHC analysis of ARF6 using anti-ARF6 antibody. ARF6 was detected in paraffin-embedded section of rat cardiac muscle tissue.