ARAF monoclonal antibody (M05), clone 3E2

• Catalog Number: orb2295914
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant ARAF.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3E2
• Tested applications: ELISA, PLA, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: ARAF (NP_001645, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: RQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGS
• NCBI: NP_001645
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ARAF monoclonal antibody (M05), clone 3E2 Western Blot analysis of ARAF expression in HeLa.

Detection limit for recombinant GST tagged ARAF is approximately 0.1 ng/ml as a capture antibody.

Proximity Ligation Analysis of protein-protein interactions between PKM2 and ARAF. HeLa cells were stained with anti-PKM2 rabbit purified polyclonal 1:1200 and anti-ARAF mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot analysis of ARAF expression in transfected 293T cell line by ARAF monoclonal antibody (M05), clone 3E2. Lane 1: ARAF transfected lysate(67.6 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (36.74 KDa).