Aquaporin 3/AQP3 Antibody

• Catalog Number: orb47955
• Category: Antibodies
• Description: Aquaporin 3/AQP3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3 (278-292aa EENVKLAHVKHKEQI), different from the related rat and mouse sequences by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, Mouse, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, HumanFlow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 31544 MW
• UniProt ID: Q92482
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Aquaporin-3;AQP-3;Aquaglyceroporin-3;AQP3;
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of rat kidney tissue using Aquaporin 3 antibody

IHC analysis of hela cell using Aquaporin 3 antibody

IHC-Fr of rat kidney tissue using Aquaporin 3 antibody

Western blot analysis of Rat Kidney Tissue Lysate(lane 1), Rat Lung Tissue Lysate(lane 2), Mouse Kidney Tissue Lysate(lane 3), MM453 Whole Cell Lysate(lane 4), SMMC-7721 Whole Cell Lysate(lane 5) using Aquaporin 3 antibody.

IHC analysis of human renal cancer tissue using Aquaporin 3 antibody.

IHC analysis of human lung cancer tissues using Aquaporin 3 antibody.

Flow Cytometry analysis of Hela cells using Aquaporin 3 antibody.