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Catalog Number | orb308768 |
---|---|
Category | Antibodies |
Description | Aquaporin 1/AQP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC, WB |
Predicted Reactivity | Bovine |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 1 (240-269aa DRVKVWTSGQVEEYDLDADDINSRVEMKPK), different from the related mouse and rat sequences by one amino acid. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Rat Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 28526 MW |
UniProt ID | P29972 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Aquaporin-1;AQP-1;Aquaporin-CHIP;Urine water chann Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of SH-SY5Y cells using anti-AQP1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of AQP1 using anti-AQP1 antibody.Lane 1:rat kidney tissue;2:mouse kidney tissue;3:mouse lung tissue.
IF analysis of AQP1 using anti-AQP1 antibody. AQP1 was detected in a paraffin-embedded section of human lung cancer tissue.
IF analysis of AQP1 using anti-AQP1 antibody. AQP1 was detected in a paraffin-embedded section of rat kidney tissue.
IHC analysis of AQP1 using anti-AQP1 antibody. AQP1 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue.
IHC analysis of AQP1 using anti-AQP1 antibody. AQP1 was detected in a paraffin-embedded section of human liver cancer tissue.
IHC analysis of AQP1 using anti-AQP1 antibody. AQP1 was detected in a paraffin-embedded section of human lung cancer tissue.
IHC analysis of AQP1 using anti-AQP1 antibody. AQP1 was detected in a paraffin-embedded section of human rectal cancer tissue.
IHC analysis of AQP1 using anti-AQP1 antibody. AQP1 was detected in a paraffin-embedded section of human tonsil tissue.
IHC analysis of AQP1 using anti-AQP1 antibody. AQP1 was detected in a paraffin-embedded section of mouse kidney tissue.
IHC analysis of AQP1 using anti-AQP1 antibody. AQP1 was detected in a paraffin-embedded section of rat kidney tissue.
FC, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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