APRT Antibody

• Catalog Number: orb1264524
• Category: Antibodies
• Description: APRT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit Ig
• Immunogen: This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-170 amino acids from the C-terminal region of human APRT.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 20 kDa
• Target: APRT
• UniProt ID: P07741
• NCBI: P07741
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Adenine phosphoribosyltransferase, APRT, APRT
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in MCF-7 cell line and rat kidney tissue lysates (35 ug/lane).

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with APRT Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.