APRIL Antibody [Sacha-2]

• Catalog Number: orb1273946
• Category: Antibodies
• Description: APRIL Antibody [Sacha-2]
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: Sacha-2
• Tested applications: ELISA, FC, ICC
• Reactivity: Human, Mouse
• Isotype: IgG2a
• Immunogen: APRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250).
• Concentration: batch dependent
• Dilution range: APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture), Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 μm sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 μg/mL for 1hr after blocking in PBS/1%BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% sodium azide) containing 1 μg/mL of Sacha-2 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• UniProt ID: O75888, Q15848
• Storage: APRIL antibody can be stored at 4°C, stable for one year.
• Buffer/Preservatives: PBS containing 0.02% sodium azide
• Note: For research use only
• Application notes: APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture), Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 μm sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 μg/mL for 1hr after blocking in PBS/1%BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% sodium azide) containing 1 μg/mL of Sacha-2 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.
• Expiration Date: 12 months from date of receipt.