APP Antibody (Center)

• Catalog Number: orb1936487
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB33592
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 86943 Da
• Target: This APP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-362 amino acids from the Central region of human APP.
• UniProt ID: P05067
• NCBI: NP_000475.1, NP_958816.1, NP_001191232.1, NP_001129601.1, NP_001129602.1, NP_001129488.1, NP_958817.1, NP_001191230.1, NP_001191231.1, NP_001129603.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Amyloid beta A4 protein, ABPP, APPI, APP, Alzheime
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

APP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of APP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

APP Antibody (Center) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the APP antibody detected the APP protein (arrow).

Confocal immunofluorescent analysis of APP Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).