Apom Antibody

• Catalog Number: orb1246450
• Category: Antibodies
• Description: Apom Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KGNMELRTEGRPD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 26kDa band observed in Human Placenta lysates (calculated MW of 21.3kDa according to NP_061286.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu and Dahlbäck, J Biol Chem. 1999 Oct 29;274(44):31286-90.; PMID: 10531326). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: Apom
• UniProt ID: Q9Z1R3
• NCBI: NP_061286.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Apom, apolipoprotein M, G3a, NG20, 1190010O19Rik
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 26kDa band observed in Human Placenta lysates (calculated MW of 21.3kDa according to NP_061286.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu and Dahlbäck, J Biol Chem. 1999 Oct 29;274(44):31286-90.; PMID: 10531326). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246450 (0.5 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246450 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.