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Catalog Number | orb381045 |
---|---|
Category | Antibodies |
Description | Apolipoprotein CIII/APOC3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human Apolipoprotein CIII recombinant protein (Position: S21-A99). Human Apolipoprotein CIII shares 55.3% and 54.3% amino acid (aa) sequence identity with mouse and rat Apolipoprotein CIII, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Mouse, Rat Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 10852 MW |
UniProt ID | P02656 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Apolipoprotein C-III;Apo-CIII;ApoC-III;Apolipoprot Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of SiHa cells using anti-Apolipoprotein CIII antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of U20S cells using anti-Apolipoprotein CIII antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Apolipoprotein CIII using anti-Apolipoprotein CIII antibody.Lane 1:rat liver tissue;2:rat RH35 cell;3:mouse liver tissue;4:mouse HEPA1-6 cell.
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