APOF Antibody

• Catalog Number: orb1246581
• Category: Antibodies
• Description: APOF Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KSYDLDPGAGSLE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 35kDa band observed in Human Liver lysates (calculated MW of 35.4kDa according to NP_001629.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows staniing of droplets in the cytoplasm of hepatocytes. Recommended concentration, 2.5-3.8ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: APOF
• UniProt ID: Q13790
• NCBI: NP_001629.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: Apolipoprotein F, APOF, Apo-F, DKFZp781G18150, MGC
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 35kDa band observed in Human Liver lysates (calculated MW of 35.4kDa according to NP_001629.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Liver shows staniing of droplets in the cytoplasm of hepatocytes. Recommended concentration, 2.5-3.8ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246581 (0.5 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246581 (3.8 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.