API5 monoclonal antibody (M01), clone 1C2

• Catalog Number: orb2291875
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant API5.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: API5 (AAH17709, 400 a.a. ~ 504 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: GEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNLGNFNYERSLQGK
• Tested applications: ELISA, IF, IHC-P, WB
• Clone Number: 1C2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH17709

API5 monoclonal antibody (M01), clone 1C2 Western Blot analysis of API5 expression in Hela S3 NE.

Detection limit for recombinant GST tagged API5 is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to API5 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to API5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Western Blot detection against Immunogen (37.18 KDa).