API5 Antibody

• Catalog Number: orb1525997
• Category: Antibodies
• Description: API5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1 and 50
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: API5
• UniProt ID: Q9BZZ5
• NCBI: XP_508375.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: Fibroblast growth factor 2-interacting factor; Pro
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse API5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-API5 antibody orb1525997 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human API5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-API5 antibody orb1525997 used for IP at 3 µg per reaction.

Detection of human API5 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. orb1525997) used at 1: 200 (1 µg/ml). Detection: DAB

Detection of mouse API5 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-API5 (Cat. No. orb1525997) used at 1: 200 (1 µg/ml) Detection: DAB