Aph-1 alpha antibody

• Catalog Number: orb666043
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Aph-1 alpha
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide of human Aph-1 alpha. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: APH1A
• Entrez: 51107, 226548
• UniProt ID: Q8BVF7, Q96BI3
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-PSF antibody, anti-Gamma-secretase subunit AP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Aph-1 alpha expression in U937 (A), HT29 (B), HeLa (C) whole cell lysates. (Predicted band size: 20; 26; 28 kD; Observed band size: 29 kD)

Immunofluorescent analysis of Aph-1 alpha staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.