APG4B (Acetyl K39) Rabbit Polyclonal Antibody

• Catalog Number: orb783001
• Category: Antibodies
• Description: APG4B (Acetyl K39) Rabbit Polyclonal Antibody
• Target: ATG4B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human APG4B(Acetyl K39)
• UniProt ID: Q9Y4P1
• MW: 44 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APG4B(Ac-Lys39); APG4B(Acetyl Lys39); APG4B(Ac-K39
• Research Area: Active Protein-Gene, Autophagy, Cancer Research, C
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (APG4B (Acetyl K39)) Polyclonal Antibody, Unconjugated (orb783001) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (APG4B (Acetyl K39)) Polyclonal Antibody, Unconjugated (orb783001) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (APG4B (Acetyl K39)) Polyclonal Antibody, Unconjugated (orb783001) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.