APAF-1 antibody

• Catalog Number: orb665869
• Category: Antibodies
• Description: Rabbit polyclonal antibody to APAF-1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Primate
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human APAF-1. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: APAF1
• Entrez: 317, 11783
• UniProt ID: O14727, O88879
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-KIAA0413 antibody, anti-Apoptotic protease-ac
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of APAF-1 expression in SHSY5Y (A), K562 (B), Jurkat (C), SP20 (D), A375 (E) whole cell lysates. (Predicted band size: 141 kD; Observed band size: 150 kD)

Immunohistochemical analysis of APAF-1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.