ANXA1 Antibody [SAIC-13B-19], Rabbit IgG

• Catalog Number: orb1672072
• Category: Antibodies
• Description: ANXA1 Antibody [SAIC-13B-19], Rabbit IgG
• Clonality: Recombinant
• Clone Number: SAIC-13B-19
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Peptide "AAYLQETGKPLDETLK" derived from Annexin A1 conjugated to KLH.
• Concentration: batch dependent
• Conjugation: Unconjugated
• UniProt ID: P04083
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: ANXA1 protein, hCG_17305
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-ANXA1 antibody SAIC-13B-19. A431(A) (0.1 ug/ml)- A549(B) (0.1 ug/ml)- HeLa(C) (0.1 ug/ml)- and K562(D) (0.3 ug/ml) cell lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-13B-19 (orb1672072)- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow cytometry using the Anti-ANXA1 antibody SAIC-13B-19. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-13B-19 (orb1672072 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-ANXA1 (SAIC-13B-19). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-13B-19 (orb1672072) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672072- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.