Anti-Vinculin Antibody

• Catalog Number: orb334810
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Vinculin
• Target: VCL
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Vinculin. The exact sequence is proprietary.
• UniProt ID: P85972, P18206, Q64727
• Tested applications: IH, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ZNF499 antibody, anti Zinc finger and BTB dom
• Note: For research use only
• Entrez: 22330, 7414, 305679

Western blot analysis of Vinculin expression in Hela (A), K562 (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 123 kD; Observed band size: 130 kD)

Immunohistochemical analysis of Vinculin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.