Vimentin Antibody

SKU: orb44574

Description

Mouse monoclonal antibody to Vimentin

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-P, WB
• Reactivity: Human
• Application Notes:
  ELISA: The antibody VI-RE/1 recognizes different epitope on human vimentin than the antibody VI-01 (IgM). Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.Immunocytochemistry: Recommended dilution: 5-10 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: VI-RE/1
• Immunogen: Bacterially expressed full-length human vimentin
• Target: Vimentin
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry analysis (intracellular staining) of vimentin expression in ESS-1 cells using anti-human vimentin (VI-RE/1) purified, GAM-FITC. Negative control: human lymphocytes.

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-RE/1 (orb44574, diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1 µg/ml; orange).

Western blotting analysis of human vimentin using mouse monoclonal antibody VI-RE/1 on lysates of MOLT-4 cell line (low expression), U87 cell line (positive) and Raji cell line (negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-vimentin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for vimentin at approximately 55 kDa.

Quick Database Links

Gene Symbol

Vimentin

UniProt

P08670

NCBI Gene ID

7431