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Item 1 of 3
Anti-VCP Purified
Catalog Number: orb43874
| Catalog Number | orb43874 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to Intra-Acrosomal Protein. |
| Target | VCP |
| Clonality | Monoclonal |
| Isotype | Mouse IgM |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Purity | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Purification | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Immunogen | Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. |
| UniProt ID | P55072 |
| RRID | AB_10993871 |
| Tested applications | FC, ICC, WB |
| Application notes | Immunocytochemistry: Recommended dilution: 10 μg/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought). Flow cytometry: Intraacrosomal staining; recommended dilution: 3-12 µg/ml. |
| Antibody Type | Primary Antibody |
| Clone Number | Hs-14 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-Intra-Acrosomal Protein (Human antibody, anti Read more... |
| Note | For research use only |
| Entrez | 7415 |
Separation of human sperm cells stained using anti-VCP (Hs-14) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from human sperm cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
Flow cytometry intracellular staining pattern of human sperm cells stained using anti-VCP (Hs-14) purified antibody (concentration in sample 9 µg/ml) GAM APC.
Immunocytochemistry analysis of VCP in acetone-permeabilized human sperms (normal spermiogram)using monoclonal antibody Hs-14 demonstrates its location to the acrosome.
