VCP Antibody

SKU: orb43874

Description

Mouse Monoclonal to Intra-Acrosomal Protein.

Research Area

Autophagic, Cancer, DNA Damage, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: FC, ICC, WB
• Reactivity: Human, Mouse
• Application Notes:
  Immunocytochemistry: Recommended dilution: 10 μg/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought). Flow cytometry: Intraacrosomal staining; recommended dilution: 3-12 µg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone No.: Hs-14
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Target: VCP
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TERA, CDC48

Separation of human sperm cells stained using anti-VCP (Hs-14) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from human sperm cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Flow cytometry intracellular staining pattern of human sperm cells stained using anti-VCP (Hs-14) purified antibody (concentration in sample 9 µg/ml) GAM APC.

Immunocytochemistry analysis of VCP in acetone-permeabilized human sperms (normal spermiogram)using monoclonal antibody Hs-14 demonstrates its location to the acrosome.

Quick Database Links

Gene Symbol

VCP

UniProt

P55072

NCBI Gene ID

7415