TRAF2 Antibody

SKU: orb1473795

Description

The TRAF2 Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human TRAF2. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant fusion protein of human TRAF2. The exact sequence is proprietary.
• Purification: This antibody is purified through a protein G column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3

Western blot analysis of TRAF2 expression in 293 (A), Hela (B) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)

Immunofluorescent analysis of TRAF2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q12933

NCBI Gene ID

7186