TNF alpha Antibody

SKU: orb323199

Description

The TNF alpha Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human TNF alpha. Purification: Affinity chromatography

Research Area

Epigenetics, NF-kB, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant protein corresponding to human TNF alpha.
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a

Western blot analysis of TNF alpha expression in recombinant human TNF-a protein (A) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 17; 25 kD)

Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P06804, P01375, P16599

NCBI Gene ID

24835, 21926, 7124

References

Sferra, Roberta et al. Prolonged Chronic Consumption of a High Fat with Sucrose Diet Alters the Morphology of the Small Intestine Int J Mol Sci, 22, 7280 (2021)

PubMed: 34298894