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TNF alpha Mouse Monoclonal Antibody
SKU: orb323199
Featured
Description
Research Area
Epigenetics, NF-kB, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:1000-1:2000, IHC-P: 1:50-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Recombinant protein corresponding to human TNF alpha. |
| Target | TNF |
| Purification | Affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
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Western blot analysis of TNF alpha expression in recombinant human TNF-a protein (A) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 17; 25 kD)
Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Sferra, Roberta et al. Prolonged Chronic Consumption of a High Fat with Sucrose Diet Alters the Morphology of the Small Intestine Int J Mol Sci, 22, 7280 (2021)
TNF alpha Mouse Monoclonal Antibody (orb323199)
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