TNF alpha Antibody

• Catalog Number: orb323199
• Category: Antibodies
• Description: Mouse monoclonal antibody to TNF alpha
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: Affinity chromatography
• Immunogen: Recombinant protein corresponding to human TNF alpha.
• UniProt ID: P01375, P16599, P06804
• Tested applications: IHC, WB
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:50-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TN
• Research Area: Epigenetics, NF-kB, Neuroscience, Signaling Pathwa
• Note: For research use only
• Entrez: 7124, 21926, 24835
• Expiration Date: 12 months from date of receipt.

References

Sferra, Roberta et al. Prolonged Chronic Consumption of a High Fat with Sucrose Diet Alters the Morphology of the Small Intestine Int J Mol Sci, 22, 7280 (2021)

PubMed: 34298894

Western blot analysis of TNF alpha expression in recombinant human TNF-a protein (A) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 17; 25 kD)

Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.