Anti-SYK Purified

• Catalog Number: orb44501
• Category: Antibodies
• Description: Mouse Monoclonal to Syk.
• Clonality: Monoclonal
• Clone Number: SYK-01
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: Recombinant fragment (aa 5-360) of human Syk.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: SYK
• Entrez: 6850
• UniProt ID: P43405
• RRID: AB_10998965
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Syk
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml; positive control: RBL rat basophilic leukemia cell line, A-431 human epidermoid carcinoma cell line, RAMOS lymphoma cell line, U-937 human histiocytic lymphoma cell line, JURKAT human peripheral blood T cell leukemia cell line; negative control: HeLa human cervix carcinoma cell line; non-reducing conditions. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: tonsil B cells.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Western blotting analysis of human Syk using mouse monoclonal antibody SYK-01 on lysates of Ramos and Jurkat (negative control) cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-Syk monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Syk was detected around 72 kDa.