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Description
Research Area
Cell Biology, Neuroscience
Images & Validation
−| Tested Applications | FC, ICC, IHC, IHC-Fr, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells |
| Reactivity | Human |
| Application Notes |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | B4D8 |
| Immunogen | E. coli-derived human Stefin B recombinant protein (Position: M1-F98). Human Stefin B shares 78.6 % amino acid (aa) sequence identity with both mouse and rat Stefin B. |
| Target | Cystatin-B |
| Molecular Weight | 14 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CPI B; CST6; CSTB; Cystatin B; cystatin B (stefin B); EPM1; PME; Stefin B; STFB
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IHC, WB
Human
Mouse
Monoclonal
Unconjugated
20 μg, 100 μg, 100 μg (without BSA and Azide)

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Quick Database Links
Gene Symbol
Cystatin-B
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry







