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Catalog Number | orb1422956 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to STAT5 (pY694/699). |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | STAT5A; STAT5B |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Porcine, Rat, Virus |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human STAT5 (pY694/699). The exact sequence is proprietary |
Dilution range | WB: WB (1/500 - 1/1000), IHC (1/100 - 1/200) |
Conjugation | Unconjugated |
UniProt ID | P42229, P51692 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide |
Alternative names | STAT5A; STAT5; Signal transducer and activator of Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of STAT5 (pY694/699) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of STAT5 (pY694/699) expression in K562 (A) whole cell lysates.