STAT3 Antibody

SKU: orb1474605

Description

The STAT3 Antibody is suitable for FC, IF, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against A synthetic peptide of human STAT3 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human STAT3
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Disclaimer: For research use only

Alternative Names

APRF; Signal transducer and activator of transcription 3; Acute-phase response factor

Western blot analysis of STAT3 expression in Jurkat (A), C6 (B), CHOK1 (C) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 88 kD)

Immunofluorescent analysis of STAT3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

UniProt

P52631, P42227, P40763

NCBI Gene ID

25125, 20848, 6774