Anti-SMAD3 (pS204) Antibody

• Catalog Number: orb1423475
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SMAD3 (pS204).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: SMAD3
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Porcine, Rat, Virus
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)
• Conjugation: Unconjugated
• UniProt ID: P84022
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: MADH3; Mothers against decapentaplegic homolog 3;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of SMAD3 (pS204) staining in HeLa Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot analysis of SMAD3 (pS204) expression in HCT116 (A), A549 (B) whole cell lysates.