SH3GL2 Antibody (monoclonal, 6I8E1)

• Catalog Number: orb1152384
• Category: Antibodies
• Description: Anti-SH3GL2 Antibody (monoclonal, 6I8E1). Tested in WB applications. This antibody reacts with Mouse, Rat.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E.coli-derived human SH3GL2 recombinant protein (Position: R65-Q292).
• UniProt ID: Q99962
• MW: 40 kDa
• Tested applications: WB
• Application notes: Western blot, 0.25-0.5 μg/ml, Mouse, Rat. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Cross Reactivity: No cross-reactivity with other proteins.
• Antibody Type: Primary Antibody
• Clone Number: 6I8E1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 3-oxo-5-beta-steroid 4-dehydrogenase; Aldo-keto re
• Note: For research use only

Western blot analysis of SH3GL2 using anti-SH3GL2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SH3GL2 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SH3GL2 at approximately 40 kDa. The expected band size for SH3GL2 is at 40 kDa.