SH3GL2 Mouse Monoclonal Antibody

SKU: orb1152384

Description

Anti-SH3GL2 Antibody (monoclonal, 6I8E1). Tested in WB applications. This antibody reacts with Mouse, Rat.

Research Area

Epigenetics & Chromatin, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: 6I8E1
• Immunogen: E.coli-derived human SH3GL2 recombinant protein (Position: R65-Q292).
• Target: Endophilin-A1
• Molecular Weight: 40 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CNSA2; EEN B1; Endophilin 1; Endophilin A1; SH3 domain GRB2 like 2; SH3 domain protein 2A; SH3D2A; SH3GL2; SH3P4

Western blot analysis of SH3GL2 using anti-SH3GL2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SH3GL2 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SH3GL2 at approximately 40 kDa. The expected band size for SH3GL2 is at 40 kDa.

Quick Database Links

Gene Symbol

Endophilin-A1

UniProt

Q99962