Anti-RSK2 Antibody

• Catalog Number: orb1474427
• Category: Antibodies
• Description: Rabbit monoclonal antibody to RSK2
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IH, IP, WB
• Reactivity: Hamster, Human
• Immunogen: A synthetic peptide of human Rsk 2/MAPKAP Kinase 1b
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: RPS6KA3
• Entrez: 6197
• UniProt ID: P51812
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: ISPK1; MAPKAPK1B; RSK2; Ribosomal protein S6 kinas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RSK2 expression in CHOK1 (A), Ramos (B) whole cell lysates. (Predicted band size: 84 kD; Observed band size: 84 kD)

Immunohistochemical analysis of RSK2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.98). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.