Anti-RAB23 Antibody

• Catalog Number: orb1473634
• Category: Antibodies
• Description: Mouse monoclonal antibody to RAB23
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein of human RAB23. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RAB23
• Entrez: 51715
• UniProt ID: Q9ULC3, P35288
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Ras-related protein Rab-23
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RAB23 expression in human brain (A), mouse brain (B), MDAMB231 (C), mouse Cerebellum (D), human Cerebellum (E) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 31 kD)

Immunofluorescent analysis of RAB23 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of U2OS cells using Anti-RAB23 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.