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Catalog Number | orb44753 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to PRR7. |
Clonality | Monoclonal |
Clone Number | TRAP3/10 |
Tested applications | ICC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG2a |
Immunogen | Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253) |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | PRR7 |
Entrez | 80758 |
UniProt ID | Q8TB68 |
RRID | AB_10993555 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | PRR7 antibody, anti-TRAP3 Read more... |
Note | For research use only |
Application notes | Immunocytochemistry: Recommended dilution: 10 μg/ml; cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100. Western blotting: Recommended dilution: 1 μg/ml; positive control: murine brain lysate (red. Laemmli buffer). |
Expiration Date | 12 months from date of receipt. |
Western blotting detection of PRR7 / TRAP3 in murine brain lysate using the monoclonal antibody TRAP3/10.
Immunocytochemistry staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).
Western blotting analysis of PRR7 using mouse monoclonal antibody TRAP3/10 on rat neuron lysate, and on HeLa transfectants overexpressing human PRR7, compared with non-transfected HeLa cells (negative control). Nitrocellulose membrane was probed with 1 µg/ml of mouse anti-PRR7 followed by a secondary antibody. PRR7 was detected around 35 kDa.