Anti-Prohibitin Antibody

• Catalog Number: orb1473909
• Category: Antibodies
• Description: Mouse monoclonal antibody to Prohibitin
• Target: PHB
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant fusion protein of human Prohibitin. The exact sequence is proprietary.
• UniProt ID: P67779, P67778, P35232
• Tested applications: IH, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Prohibitin
• Note: For research use only
• Entrez: 18673, 5245, 25344

Western blot analysis of Prohibitin expression in 293 (A), MCF7 (B), HepG2 (C), NIH3T3 (D), rat liver (E) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 30 kD)

Immunohistochemical analysis of Prohibitin staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.