Anti-PRKAR1A Antibody

• Catalog Number: orb1423421
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRKAR1A.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: PRKAR1A
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Porcine, Rat, Virus
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Conjugation: Unconjugated
• UniProt ID: P10644
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PKR1; PRKAR1; TSE1; cAMP-dependent protein kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of PRKAR1A staining in HT29 Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of PRKAR1A expression in HEK293T (A), MCF7 (B), mouse testis (C), rat testis (D) whole cell lysates.