Anti-PON1 Antibody (monoclonal, 9D3)

• Catalog Number: orb421113
• Category: Antibodies
• Description: Anti-PON1 Antibody (monoclonal, 9D3). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9D3
• Tested applications: ELISA, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: E. coli-derived mouse PON1 recombinant protein (Position: A30-D274).
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 40 kDa
• UniProt ID: P52430
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Serum paraoxonase/arylesterase 1; PON 1
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml ELISA (Cap), 1-5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Sandwich ELISA - Recombinant mouse PON1 protein standard curve. Use in combination with reagents from Mouse PON1 ELISA Kit EZ-Set (DIY Antibody Pairs).

Western blot analysis of PON1 using anti-PON1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PON1 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.