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Anti-PKC iota/zeta (pT412/410) Antibody

Catalog Number: orb737126

DispatchUsually dispatched within 2-3 weeks
$ 600.00
Catalog Numberorb737126
CategoryAntibodies
DescriptionRabbit polyclonal antibody to PRKCI
Species/HostRabbit
ClonalityPolyclonal
Clone NumberPRKCI; PRKCZ
Tested applicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Dilution rangeWB: 1/500 - 1/1000, IF: 1/50 - 1/200
ConjugationUnconjugated
UniProt IDP41743
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesAnti-DXS1179E antibody, anti-Protein kinase C iota
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-PKC iota/zeta (pT412/410) Antibody

Western blot analysis of PKC iota/zeta (pT412/410) expression in Hela (A), mouse lung (B) whole cell lysates.

Anti-PKC iota/zeta (pT412/410) Antibody

Immunohistochemical analysis of PKC iota/zeta (pT412/410) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-PKC iota/zeta (pT412/410) Antibody

Immunofluorescent analysis of PKC iota/zeta (pT412/410) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.