PI 3 Kinase Class 3 PIK3C3 Rabbit Monoclonal Antibody

SKU: orb548012

Description

Anti-PI 3 Kinase Class 3 PIK3C3 Rabbit Monoclonal Antibody. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Immunology & Inflammation, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB 1:500-1:2000 IHC 1:50-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: CDO-16
• Immunogen: A synthesized peptide derived from human PI 3 Kinase Class 3
• Target: Phosphatidylinositol 3-kinase catalytic subunit type 3
• Molecular Weight: 100 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3; 2.7.1.137; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34; PIK3C3; VPS34

Immunohistochemical analysis of paraffin-embedded human kidney, using PI 3 Kinase Class 3 Antibody (orb548012) PIK3C3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PIK3C3 Antibody (orb548012) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Western blot analysis of PI 3 Kinase Class 3 expression in 293T cell lysate (orb548012). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3C3 monoclonal antibody (Catalog # orb548012) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for PIK3C3.

Quick Database Links

Gene Symbol

Phosphatidylinositol 3-kinase catalytic subunit type 3

UniProt

Q8NEB9