Perforin Antibody

SKU: orb222761

Description

Mouse monoclonal antibody to Perforin

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IHC-P, IP
• Reactivity: Bovine, Human
• Application Notes:
  Flow cytometry: Recommended dilution: 3-12 μg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Clone No.: dG9
• Immunogen: purified granules from human YT lymphoma cell line
• Target: Perforin
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

PRF1, P1, PFP, HPLH2

Separation of perforin positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using anti-human perforin (dG9) purified antibody (concentration in sample 2 μg/ml, GAM FITC).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Perforin (dG9) purified antibody (concentration in sample 2 μg/ml, GAM FITC).

Quick Database Links

Gene Symbol

Perforin

UniProt

P14222

NCBI Gene ID

5551